My Interest Biology Experiment - 3

The name of the experiment : Pigment Chromatography

1. What is Chromatography?

-> The basic meaning of Chromatography means the method used in laboratories in order to separate different mixtures. How this works is using the time difference for different mixture to climb up a certain material.
So by plunging the material used for chromatography into a certain liquid and dabbing the mixed mixture at the bottom the liquid begins to climb up the material. In addition to this phenomenon the dabbed mixture begins to climb also with each differential speed. So in the end after waiting long enough  until there is no more climbing, we can see that the mixtures have been separated due to the differential time needed for the mixture to climb.

2. Experiment No.1

< White Royal Azalea >
- Collect five blossoms of this flower
- Remove the pistil, stamen, and calyx and grind the remaining flowers.
- Add in some liquid with the mixture of 1 : 3 ratio to Aceton and Methanol and mix it well
- Extract the liquid mixed with the ground flower and put it in a centrifuge.
- After the centrifuge, extract the liquid and dab it to your material for Chromatography.
- Place the material into a substance and wait.
- After a while examine it withe bare eyes.
- After that examine it with ultraviolet light.

3. Experiment No.2

< Reddish Royal Azalea >
- Collect five blossoms of this flower
- Remove the pistil, stamen, and calyx and grind the remaining flowers.
- Add in some liquid with the mixture of 1 : 3 ratio to Aceton and Methanol and mix it well
- Extract the liquid mixed with the ground flower and put it in a centrifuge.
- After the centrifuge, extract the liquid and dab it to your material for Chromatography.
- Place the material into a substance and wait.
- After a while examine it withe bare eyes.
- After that examine it with ultraviolet light.

4. Experiment No.3

< Yellow Daisy >
- Collect 5 blosom of this
- This time removing the pistil, stamen, and calyx is impossible for the parts are all too small.
- Add in some liquid with the mixture of 1 : 3 ratio to Aceton and Methanol and mix it well

- Extract the liquid mixed with the ground flower and put it in a centrifuge.
- After the centrifuge, extract the liquid and dab it to your material for Chromatography.
- Place the material into a substance and wait.
- After a while examine it withe bare eyes.
- After that examine it with ultraviolet light.

5. Analyze and Conclusion

1) Royal Azalea has some highlighting pigments in it's components .
2) On the other hand Daisy doesn't have the factor needed to light up the chromatography in the ultraviolet light.

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<Things that could have been improved>

1) Taking a more careful attention to the amount of liquids used in the experiment in order for the experiment to have more higher accuracy.

THE END!!!